2017-2018年我国部分地区牛支原体的分离鉴定及多位点序列分型

doi:10.11843/j.issn.0366-6964.2019.09.013

摘要/Abstract

摘要： 旨在分离流行于我国部分地区的牛支原体，掌握我国牛支原体的种群结构和进化关系。本研究于2017-2018年采集黑龙江、河北、江苏、安徽、内蒙古等地规模化养殖场327份病牛样品，分离鉴定出20株牛支原体。采用adh1、gltX、gpsA、gyrB、pta2、tdk、tkt 7个管家基因对分离株进行多位点序列分型（MLST），分析我国牛支原体种群结构并结合GenBank数据库公布的85株牛支原体进行最小生成树（MST）分析。结果显示，MycbPAN005为ST-6型，其余菌株均为ST-10型，分析表明ST-10型为我国的主要流行型。我国牛支原体属于CC1克隆复合体，且除ST-6型外，ST-32、ST-26和ST-43型与ST-10型仅有一个管家基因存在差异，表明我国牛支原体种群结构相对稳定单一。ST-6型中国株MycbPAN005为国内首次报道，可为牛支原体的流行病数据库研究提供新的资料。

Abstract: The purpose of this study is to isolate Mycoplasma bovine (M. bovis) epdemic strains in some areas of China and analyse the genetic characteristics and evolutionary relationship of M. bovis. Three hundred and twenty-seven samples were collected from cattle with respiratory symptoms in several large-scale farms of Heilongjiang, Hebei, Jiangsu, Anhui and Inner Mongolia between 2017 and 2018. Twenty strains of M. bovis were cultured and identified by 16S rRNA sequencing. The isolates were classified according to Multiple Locus Sequence Typing (MLST) with the adoption of 7 housekeeping genes including adh1,gltX,gpsA,gyrB,pta2,tdk and tkt. In order to analyze the genetic characteristics and evolutionary relationship of M. bovis isolates, the minimum spanning tree of 20 clinical isolates and 85 strains of M. bovis published in GenBank database were set up. The results showed that MycbPAN005 was identified as ST-6 and the other strains were identified as ST-10, indicating that ST-10 was the main epidemic type in China. All M. bovis in China belong to CC1 category and there is only one housekeeping gene different among ST-32, ST-26, ST-43 and ST-10 except for ST-6, which indicate that the population structure of M. bovis is relatively steady in China.This is the first report domestically about ST-6 strain, providing new materials for epidemic disease database research of M. bovis.

中图分类号:

S852.62

康浩然, 刘重阳, 于勇, 张俊杰, 潘子豪, 姚火春. 2017-2018年我国部分地区牛支原体的分离鉴定及多位点序列分型[J]. 畜牧兽医学报, 2019, 50(9): 1857-1863.

KANG Haoran, LIU Chongyang, YU Yong, ZHANG Junjie, PAN Zihao, YAO Huochun. Isolation,Identification and Multilocus Sequence Typing Analysis of Mycoplasma bovis Strains in Some Regions of China during 2017 to 2018[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1857-1863.

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